AVS 52nd International Symposium
    Biomaterial Interfaces Monday Sessions
       Session BI-MoP

Paper BI-MoP33
Electrical Monitoring of Cell Interaction on a Microelectronic Interface

Monday, October 31, 2005, 5:00 pm, Room Exhibit Hall C&D

Session: Biomaterial Interfaces Poster Session
Presenter: K. Dominizi, Vienna University of Technology, Austria
Authors: H.D. Wanzenboeck, Vienna University of Technology, Austria
K. Dominizi, Vienna University of Technology, Austria
P. Hagl, Vienna University of Technology, Austria
E. Bertagnolli, Vienna University of Technology, Austria
E. Bogner, University Vienna, Austria
F. Gabor, University Vienna, Austria
M. Wirth, University Vienna, Austria
Correspondent: Click to Email
*****PLEASE NOTE: YOU MUST IDENTIFY A DIFFERENT PRESENTER FOR THIS ABSTRACT. YOU MAY ONLY PRESENT ONE (1) PAPER AT THE CONFERENCE*****The electrical measurement of tissue properties and of cell signals has gained increased interest for ceel-based biosensor applications in medicine, pharmacology and biology. Yet, the interaction of living cells on solid sensor surfaces has not been thoroughly investigated. For application mainly microelectronic sensors are attractive due to the small size and the low cost in mass production. The objective of this work was to investigate the interaction of living human cells with microelectronic surfaces. We have performed a comprehensive study of the cells behaviour on semiconductors, metals and dielectric materials commonly used in microelectronics. Human ephitelal cells (Caco-2) were grown in-vitro on the surface of the microelectronic substrates. In a second step we have systematically varied the geometry of the surface by etching trenches with a width from 2 up to 60 µm and a depth of 2 to 30 µm into a biocompatible substrate. The growth of ephitelial cells on flat and on ridged surfaces was compared. The response of the cell behaviour on the varying surface was investigated by optical, electronoptical, enzymatic and biochemical methods. The effects of surface alterations on the proliferation rate, the cell adhesion, the cell coverage and the differentiation of cells was investigated. Finally, a microelectrode structure with microelectrodes (2x2 µm2) smaller than a single cell was implemented on the previously investigated surfaces. The electrical properties of the cell layer and of single cells could be evaluated by impedance spectroscopy. Differences of the impedance were monitored during progressing aging of cells. The interpretation of the interrelated analysis results shines a new light on the interaction of living cells with sensor interfaces. The feasibility to identify changes of the cell-surface interaction by electrical measurements was successfully demonstrated.