AVS 52nd International Symposium
    Biomaterial Interfaces Monday Sessions
       Session BI-MoP

Paper BI-MoP30
ToF-SIMS Chemical Imaging Analysis of Micropatterned Streptavidin and Cells without Labeling

Monday, October 31, 2005, 5:00 pm, Room Exhibit Hall C&D

Session: Biomaterial Interfaces Poster Session
Presenter: T.G. Lee, Korea Research Institute of Standards and Science (KRISS)
Authors: T.G. Lee, Korea Research Institute of Standards and Science (KRISS)
H.K. Shon, Korea Research Institute of Standards and Science (KRISS)
K.-B. Lee, Korea Advanced Institute Science and Technology (KAIST)
J. Kim, Sungkyunkwan University, Korea
J.C. Lee, Samsung Advanced Institute of Technology (SAIT), Korea
I.S. Choi, Korea Advanced Institute Science and Technology (KAIST)
D.W. Moon, Korea Research Institute of Standards and Science (KRISS)
Correspondent: Click to Email
In this work, three different analysis ion beams (Ga@super +@, Au@super +@ and Au@sub 3@@super +@) were used to obtain label-free time-of-flight secondary ion mass spectrometry (ToF-SIMS) chemical images of microcontact printed streptavidin. The image of total ions obtained by an Au@sub 3@@super +@ primary ion beam corresponded well to the real image of micropatterned streptavidin, whereas the total-ions image by Ga@super +@ or Au@super +@ primary ion beams did not. A principal component analysis (PCA) of ToF-SIMS data was initially performed to identify characteristic secondary ions of streptavidin. Chemical images of each characteristic ion were reconstructed from raw data and used for the 2nd PCA run, which resulted in a contrasted, and corrected, image of micropatterned streptavidin by Ga@super +@ and Au@super +@ ion beams. This suggests that ToF-SIMS imaging along with multivariate data analysis would be an effectual method of obtaining label-free chemical images of patterned proteins or biomolecules. Label-free chemical images of micropatterned A431 cells were obtained by using the same procedure.