AVS 52nd International Symposium
    Biomaterial Interfaces Monday Sessions
       Session BI-MoP

Paper BI-MoP25
Vacuum UV to Mid-Infrared Optical Study of Immunoglobulin G Attachment to Chemical Modifications of Chitosan

Monday, October 31, 2005, 5:00 pm, Room Exhibit Hall C&D

Session: Biomaterial Interfaces Poster Session
Presenter: W.H. Nosal, University of Nebraska-Lincoln
Authors: W.H. Nosal, University of Nebraska-Lincoln
S. Sarkar, University of Nebraska-Lincoln
D.W. Thompson, University of Nebraska-Lincoln
A. Subramanian, University of Nebraska-Lincoln
J.A. Woollam, University of Nebraska-Lincoln
Correspondent: Click to Email
Optical constants of spin-cast chitosan films were determined from vacuum-ultraviolet (VUV) to mid-infrared (130 nm to 30 microns). Both pure and chemically modified chitosan films were studied using spectroscopic ellipsometry (SE). Chemical modification by attachment to the amine group in chitosan was performed using 1,2 Epoxy-3-phenoxy-propane, commonly known as glycidyl phenyl ether (GPE), to produce a hydrophobic surface. A similar modification with succinic anhydride yields a hydrophilic surface. Quantitative lineshape analysis of the optical constant spectra was performed using Lorentzian and Gaussian line-shapes, including anisotropic response due to molecular-bond orientations in-plane and out-of-plane. Dynamic in situ visible SE has been used to study the attachment of immunoglobulin G to each modified surface type (hydrophobic/hydrophilic), followed by VUV to mid-infrared ex situ SE optical analysis. Research supported by U. S. Army contract # W911NF-04-2-0011, and the College of Engineering, University of Nebraska.