AVS 52nd International Symposium
    Biomaterial Interfaces Monday Sessions
       Session BI-MoP

Paper BI-MoP22
Role of the Chemical and Morphological Surface Properties in Platelet Binding and Protein Adsorption to Biomaterial Surfaces

Monday, October 31, 2005, 5:00 pm, Room Exhibit Hall C&D

Session: Biomaterial Interfaces Poster Session
Presenter: M. Anderle, ITC-irst, Italy
Authors: S. Forti, ITC-irst, Italy
M. Vinante, ITC-irst, Italy
L. Pasquardini, ITC-irst, Italy
L. Lunelli, ITC-irst, Italy
L. Vanzetti, ITC-irst, Italy
R. Canteri, ITC-irst, Italy
C. Pederzolli, ITC-irst, Italy
M. Anderle, ITC-irst, Italy
S. Pascale, Sorin Biomedica Cardio S.p.A, Saluggia (VC), Italy
G. Rossetti, S. Chiara Hospital, Italy
Correspondent: Click to Email
Many existing medical implants are associated with poor interfacial biocompatibility. An example is represented by the cardiovascular devices; their implantation induces a complex blood-material interaction, often leading to thrombus formation. This work describes the characterization of the protein layer and the process of platelets adhesion on four different materials (Sorin pyrolytic carbon (PyC), two different types of polystyrene and titanium alloy) after contact with human platelet poor plasma and platelet rich plasma in static conditions. Total protein quantification on eluted samples revealed that PyC adsorbed the lowest amount of plasma proteins. Using immunofluorescence microscopy specific proteins promoting platelets adhesion were characterized on PyC and titanium alloy: fibronectin was found to be present at very low levels on both surfaces while fibrinogen and von Willebrand factor adhered to PyC in a higher proportion. Adherent platelets and shape categories distribution were quantified using scanning electron microscopy (SEM) and atomic force microscopy (AFM). PyC induced less adhesion with mostly weak activated platelets, however aggregates may be present. Titanium alloy promoted a higher adhesion, with more active platelets but less cohesive. Polystyrene materials were almost covered by spread platelets. Material surface properties were evaluated by contact angle, electron spectroscopy for chemical analysis (ESCA), secondary ion mass spectrometry (SIMS) and atomic force microscopy (AFM). The final goal will be to correlate the biological response with surface morphological and physico-chemical properties of the materials.@footnote 1@ @FootnoteText@ @footnote 1@Supported by the Provincia Autonoma di Trento, post-doc project Emosurf and by National Department of Health.