AVS 52nd International Symposium
    Biomaterial Interfaces Monday Sessions
       Session BI-MoP

Paper BI-MoP15
Protein Adsorption on Poly(Ethylene Glycol)-Modified Surfaces under Flow Conditions in Microfluidics Systems

Monday, October 31, 2005, 5:00 pm, Room Exhibit Hall C&D

Session: Biomaterial Interfaces Poster Session
Presenter: C.J. Chun, University of Central Florida
Authors: C.J. Chun, University of Central Florida
K. Lenghaus, University of Central Florida
D.C. Henry, Clemson University
L. Riedel, University of Central Florida
A. Bhalkikar, University of Central Florida
J.J. Hickman, University of Central Florida
Correspondent: Click to Email
In the last decade microfabrication technology has been used to create new microfluidics systems, bioanalytical and medical devices. The handling of relatively small amounts of analytes, at significantly lower concentrations, combined with the fact that the surface-to-volume ratio increases in direct proportion to the device size decreasing, could create potential problems in device utilization. The problem being that, the analytes or target molecules may be completely non-specifically adsorbed on the surfaces of the microdevices before they reach the detector. Thus, the basic understanding of an adsorption behavior of biomolecules@footnote 1@ on the surfaces of these systems is critical for their use in microfluidics as well as bioanalytical devices. To investigate the situation we have developed assays@footnote 2@ to evaluate protein adsorption under flow and static conditions at submonolayer coverages on poly(ethylene glycol) (PEG)-modified surfaces, which are well known to resist protein adsorption.@footnote 3@ In this study, protein adsorption onto PEG-modified microcapillary surfaces, under flow conditions, has been determined at different flow rates as well as various protein concentrations. Alkaline phosphatase and horseradish peroxidase were used to evaluate proteins adsorption behavior, which although low, was still significant. The flow rate was also seen to affect the protein adsorption on the PEG-modified-surfaces. Simulation studies of the protein adsorption behavior are also being used to help in the development of new biocompatible microfluidics systems.@footnote 4@. @FootnoteText@ @footnote 1@K. Nakanishi, T. Sakiyama, and K. Imamura, J.Biosci.Bioeng., 91, 233 (2001). @footnote 2@K. Lenghaus, J.W. Dale, J.C. Henderson, D.C. Henry, E.R. Loghin, and J.J. Hickman, Langmuir, 19, 5971 (2003). @footnote 3@M. Zhang, T. Desai, and M. Ferrari, Biomaterials, 19, 953 (1998). @footnote 4@J. Jenkins, B. Prabhakarpandian, K. Lenghaus, J.J. Hickman, and S. Sundaram, Anal.Biochem., 331, 207 (2004).