AVS 50th International Symposium
    Homeland Security Topical Conference Wednesday Sessions
       Session HS+MM-WeA

Paper HS+MM-WeA7
DIOS-MS and LC-DIOS-MS for Chemical Analysis

Wednesday, November 5, 2003, 4:00 pm, Room 309

Session: Detection of Explosives and Other Chemicals for Homeland Security
Presenter: J.E. Crowell, University of California, San Diego
Authors: Z. Shen, University of California, San Diego
C. Fish, University of California, San Diego
G. Siuzdak, The Scripps Research Institute
M.G. Finn, The Scripps Research Institute
J.E. Crowell, University of California, San Diego
Correspondent: Click to Email
Desorption/Ionization On Silicon Mass Spectrometry (DIOS-MS) is a new mass spectrometry strategy based on pulsed laser desorption/ionization from a porous silicon surface. DIOS-MS is similar to matrix-assisted laser-desorption ionization mass spectrometry (MALDI-MS) in that it utilizes the same instrument; however, in DIOS-MS, porous silicon is used to trap analytes deposited on the surface and laser radiation is used to vaporize and ionize these molecules, without the presence of any matrix material. We have shown that DIOS-MS can be used for a wide range of biomolecules, organic molecules, and metal-containing compounds at the femtomole and attomole level with little or no fragmentation. DIOS-MS offers many unique advantages including good sensitivity, low background ion interference, and high salt tolerance. We will discuss the coupling of liquid chromatography separation with DIOS-MS for protein identification and peptide sequencing, and the use of chemical and surface modification for tailored analysis.