AVS 50th International Symposium
    Biomaterial Interfaces Wednesday Sessions
       Session BI-WeP

Paper BI-WeP12
DNA-Based Protein Immobilization vs. Biotin/Streptavidin Bridges

Wednesday, November 5, 2003, 11:00 am, Room Hall A-C

Session: Poster Session
Presenter: C.L. Boozer, University of Washington
Authors: C.L. Boozer, University of Washington
J. Ladd, University of Washington
Q. Yu, University of Washington
S. Chen, University of Washington
J. Homola, Institute of Radio Engineering and Electronics, Czech Republic
S. Jiang, University of Washington
Correspondent: Click to Email
A new DNA-based protein immobilization method has been developed for use with SPR biosensors. This DNA-based immobilization method provides a convenient and versatile alternative to the commonly used biotin/streptavidin platform, with comparable, if not better, sensitivity. This work presents a comparison of these two platforms, focusing on the detection of hCG as a model system. Our results show that the DNA-based method allows for detection of lower hCG concentrations. Extensive control experiments have been performed to check both sensor platforms for non-specific binding and cross reactivity. In addition to the increased sensitivity, the DNA-based protein immobilization offers many other advantages crucial to biosensor development that the biotin/streptavidin platform does not have. While both the biotin/streptavidin complex and the DNA-based approach are robust and highly specific, the DNA based approach is much more versatile.