AVS 50th International Symposium
    Biomaterial Interfaces Tuesday Sessions
       Session BI-TuP

Paper BI-TuP9
Protein Immobilization for Multi-Channel Sensor Detection

Tuesday, November 4, 2003, 5:30 pm, Room Hall A-C

Session: Poster Session
Presenter: J. Ladd, University of Washington
Authors: J. Ladd, University of Washington
Q. Yu, University of Washington
S. Chen, University of Washington
J. Homola, Institute of Radio Engineering and Electronics, Czech Republic
S. Jiang, University of Washington
Correspondent: Click to Email
The simultaneous detection of multiple analytes is an important consideration for the advancement of current biosensor technology. Currently, few sensor systems possess the capability to accurately and precisely detect multiple antigens. The work presented demonstrates a novel approach for the functionalization of sensor surfaces for multi-channel detection. This approach combines inkjet-printing technology with self-assembled monolayer (SAM) chemistry to create a protein array. A modified commercial Epson C40UX inkjet printer is used in this work. The sensor platform is based on a layer of streptavidin immobilized on a mixed SAM of biotinylated alkanethiol (BAT) and poly(ethylene oxide) (PEO). Non-specific binding, and thus false positives, are minimized with the non-fouling background of the sensor surface. The described platform is used in a home-built surface plasmon resonance (SPR) biosensor. Results show excellent specificity in protein immobilization to the proper locations in the array, eliminating the possibility of a false detection within a channel. Analysis of multiple proteins in solution shows a similar behavior and response to pure protein solutions. The detection capabilities of a sensor using this protein array have been characterized using human choriogonadotropin (hCG).