AVS 50th International Symposium
    Biomaterial Interfaces Tuesday Sessions
       Session BI-TuP

Paper BI-TuP4
Patterned Immobilization of Proteins on High-density PEG Coated Si Surfaces

Tuesday, November 4, 2003, 5:30 pm, Room Hall A-C

Session: Poster Session
Presenter: Y. Jun, University of Minnesota
Authors: Y. Jun, University of Minnesota
X.-Y. Zhu, University of Minnesota
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Spatially localized patterns of chemical and biological functions on solid surfaces are of great interest to a number of research fields, such as biochips and cell biology. We have developed a novel chemical strategy for the formation of micro-scale patterns of chemical and biological functions on silicon surfaces using soft lithography techniques. This is based on an efficient reaction between alcohol functional groups and chlorine terminated silicon surfaces. The features formed by soft lithography show exceptionally high resolution and fidelity, with edge resolution as high as 10 nm. We demonstrate the immobilization of protein molecules to spatially localized surface functional groups on high-density PEG brush coated silicon. The spatially localized activation of the PEG brush is achieved via either partial oxidation to form aldehyde groups or via attachment of efficient leaving groups. Protein molecules are covalently immobilized to these activated regions on the PEG/Si surface.