AVS 50th International Symposium
    Biomaterial Interfaces Tuesday Sessions
       Session BI-TuM

Paper BI-TuM9
Molecule Specific Imaging Analysis of Carcinogens in Breast Cancer Cells using Time-of-Flight Secondary Ion Mass Spectrometry

Tuesday, November 4, 2003, 11:00 am, Room 307

Session: Cell/Surface Interactions
Presenter: K.J. Wu, Lawrence Livermore National Laboratory
Authors: K.J. Wu, Lawrence Livermore National Laboratory
J.N. Quong, Lawrence Livermore National Laboratory
M.G. Knize, Lawrence Livermore National Laboratory
K.S. Kulp, Lawrence Livermore National Laboratory
Correspondent: Click to Email
The concentration and localization of intracellular chemical compounds such as pharmaceuticals and carcinogens are important, specifically for application in physiology and medicine. Cooked muscle meats contain small amounts of rodent carcinogens belonging to the heterocyclic amine class of compounds and are implicated in human cancers at various organ sites. 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) is the most mass abundant in cooked foods. PhIP is a genotoxic carcinogen, which has been demonstrated to cause dose-dependent mammary and prostate tumor formation in rats. In this report we will present the results on time-of-flight secondary ion mass spectrometry (TOF-SIMS) studies of several MCF7 line of human breast cancer cells. Protocols for high vacuum compatible tissue and cell culture preparation have been developed. Such direct imaging approach permits an acquisition of element and molecule-specific images directly from the cell surface. The results show the intracellular concentration and distribution of low level carcinogenic compounds such as PhIP in flash-frozen MCF7 cells; the time dependent effects of heterocyclic amine carcinogens interaction with MCF7 cells. We will discuss two major efforts to further the imaging mass spectrometry applications for biological samples: ME-SIMS approach to enhance the ionization yields and multivariate analysis data reduction technique for compound distribution on cell surfaces. @FootnoteText@ This work was performed under the auspices of the U.S. Department of Energy by the University of California, Lawrence Livermore National Laboratory under contract No. W-7405-Eng-48.