Invited Paper BI-ThM6
Multi-technique Studies of Bio-Interface Processes; QCM-D, (Nanoparticle) SPR, SERS, AFM, Electrical Impedance, and Cell Force Sensor
Thursday, November 6, 2003, 10:00 am, Room 318/319
Title: Multi-technique studies of Bio-Interface kinetics with QCM-D,(nanoparticle) SPR, SERS, AFM, Electrical Impedance, and Cell Force Sensor. Text: Bio-interface sensing is commonly based on immobilization of some sensing molecules on a surface, and detection of how unknown sample molecules bind specifically to them. Techniques for detection include optical, electrical, mechanical and electro-acoustic methods. The first part of this talk describes the preparation of a sensing platform based on functionalized supported phospholipid bilayers, using QCM-D, AFM, SPR and electrical impedance (ac forward transmission factor).@footnote 1, 2@ Sensing applications with this platform are illustrated by several examples;@footnote 3@ DNA immobilization and hybridization, protein-bilayer interactions, and enzymatic reactions. With the QCM-D technique rich information is obtained through frequency and dissipation shift measurements at multiple (overtone) frequencies, additionally strengthened by combining this technique with SPR. The second part describes nano- and micro fabricated structures for sensing, including EBL fabricated Ag arrays for (G)SERS,@footnote 4@ colloidal lithography of nanoparticles for plasmon resonance enhancement,@footnote 5@ and standing cantilever arrays for cell force sensing.@footnote 6@ @FootnoteText@ @footnote 1@ Reimhult, E., et al., Langmuir 19 (2003) 1681-1691. @footnote 2@ Hook, F., et al., Analytical Chemistry 73 (2001) 5796-5804@footnote 3@ Hook, F., et al., Langmuir 17 (2001) 8305-8312. @footnote 4@ Gunnarsson, L., et al., NanoStructured Materials 12 (1999) 783-788.@footnote 5@ Hanarp, P., et al., Colloids and Surfaces A: Physicochemical and Engineering Aspects 214 (2003) 23-26@footnote6@ Petronis, S. et al Journal of Micromechanics and Microengineering (to be published).