AVS 50th International Symposium
    Biomaterial Interfaces Wednesday Sessions
       Session BI+SS-WeA

Paper BI+SS-WeA8
Interfacial Engineering for Protein Biochips in Proteomics Applications

Wednesday, November 5, 2003, 4:20 pm, Room 307

Session: Biomolecular Surface Science and Microfluidics
Presenter: H. Lu, Zyomyx, Inc.
Authors: H. Lu, Zyomyx, Inc.
P. Kernen, Zyomyx, Inc.
P. Wagner, Zyomyx, Inc.
Correspondent: Click to Email
Proteomics is increasingly dependent on analytical tools that focus on quantification of protein expression, biomolecular-protein interactions, and functional activity. The large numbers of proteins and complexity involved in proteomics applications present tremendous challenges for the development of analytical platforms and specifically interfacial engineering schemes. Zyomyx has developed a novel protein biochip platform that facilitates rapid, precise, highly multiplexed analysis with minimal sample requirements and has integrated several sophisticated interfacial engineering strategies. The biochip architecture consists of a three-dimensional array structure designed to provide consistent feature size and defined placement, while eliminating spot-to-spot cross contamination. We will focus on details of biochip development with an emphasis on organic layer compositions for optimal packing density, molecular orientation, selective immobilization of capture reagents, and low non-specific protein adsorption. Examples on high-level quantitative protein analysis will focus on Zyomyx human Cytokine Biochip capable of fully multiplexed and quantitative protein analysis based on sandwich-immunoassay configurations.