AVS 50th International Symposium
    Biomaterial Interfaces Friday Sessions
       Session BI+PS-FrM

Paper BI+PS-FrM3
Investigation of Organic Monomers in Plasma-induced Chemical Micropatterning

Friday, November 7, 2003, 9:00 am, Room 318/319

Session: Plasma Methods for Bio-interfaces
Presenter: G.Sh. Malkov, Colorado State University
Authors: G.Sh. Malkov, Colorado State University
M.L. Godek, Colorado State University
D.W. Grainger, Colorado State University
E.R. Fisher, Colorado State University
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Plasma-enhanced chemical vapor deposition (PE-CVD) of organic films is a valuable technique for the surface modifications of polymeric biomaterials. Recently, plasma-based methods have been developed for the fabrication of chemical micropatterns, which have a number of applications, including production of multianalyte biosensors, diagnostic tests, DNA microchips, and genomic arrays. The generation of micropatterns by means of the plasma deposition of organic compounds through a transmission electron microscope (TEM) grid mask has been reported.@footnote 1@ Here, we have created various high fidelity micron-scale patterns of different chemistries using inductively coupled pulsed RF plasma deposition through a TEM grid with the following monomers: acrylic acid, N-vinyl-2-pyrrolidinone, 2-hydroxyethyl methacrylate, N-vinylformamide, allylamine, and hexylamine on PS coated with plasma deposited, highly hydrophobic fluorocarbon materials. SEM images of the patterned surfaces will be demonstrated. Physico-chemical properties of deposited polymeric materials were characterized using angle-resolved XPS, FTIR, spectroscopic ellipsometry, and static contact angle measurements on unpatterned samples, which were plasma treated under identical plasma conditions. Directed cell attachment studies have also been performed. NIH 3T3 fibroblast cells were used to test the cell adhesion and viability on the various patterned surfaces. PS coated with FC is biologically inert: cells do not adhere on this surface. In contrast, cells proliferate well on surfaces functionalized with organic monomers. Other cell culture experiments and biomolecule patterning will be discussed. @FootnoteText@@footnote 1@ N.A. Bullet, R.D.Short et al. Surface and Interface Analysis. 2001, 31, 1074-1076.