Nanostructures with micrometer or nanometer spacings have been prepared through pure self-assembly of diblock copolymer micelles (formation and compartmental localization of metallic nanodots within block copolymer micelles) or in combination with a top-down approach (electron beam lithography). Within these structures, 7 nm Au particles can be positioned with a precision of < 10 nm and large freedom in pattern choice (periodic, aperiodic, dotes, lines). Specifically, this is obtained by casting a solution of HAuCl4 loaded block copolymer micelles onto the prestructured resist film. Due to capillary effects and steric hindering, the particles are centered within the prepatterned holes and at the edges of prestructured lines. Subsequent lift-off of the resist allows the removal of all micelles with the exception of those that are in direct contact to the underlying substrate. The block copolymer is then removed by plasma etching, which strips the polymer micelle and reduces the gold salt to gold thereby leaving behind nanoscopic dots or lines of gold in a defined array. These nanostructured interfaces are used as platform for biofunctionalisation of solid interfaces. The surfaces are used as a tool to investigate cluster formation of focal adhesion associated proteins of fibroblasts. Cultured human melanocytes allowed to study the regulation of cell shape through contact with interfaces offering different topography and biochemical pattern. The cellular morphology of melanocytes is a measurable indicator for cell reaction to the cellular environment. The characteristic cellular shape of different cell cultures was quantified by different shape parameters like the number and length of dendrites. A decreased signal-to-noise ratio was found for melanocyte cells concerning the number of dendrites and orientation of dendrites if cultured on biochemically and topographically structured substrates.