AVS 49th International Symposium
    Biomaterials Wednesday Sessions
       Session BI-WeP

Paper BI-WeP8
New Diazonium-Functionalized Support for Fabrication of Protein Microarrays

Wednesday, November 6, 2002, 11:00 am, Room Exhibit Hall B2

Session: Biointerfaces and Surfaces II
Presenter: Y. Wu, University of New Mexico
Authors: Y. Wu, University of New Mexico
G.P. Lopez, University of New Mexico
Correspondent: Click to Email
Microarray technologies have rapidly become a major trend in high-throughput functional genomic study since its birth at the early 1990s. Recent advances of this technology have been focused on high-throughput proteomic analysis. The difficulty in immobilizing proteins onto solid surfaces without denaturation has led to the search for new general methods for coupling proteins to solid substrates. To this end, a chemical process for covalently linking proteins onto an ordinary microscope slide in a manner that preserves the ability of the immobilized proteins to interact with other proteins has been studied. The method uses p-aminophenyl trimethoxysilane (ATMS) /diazotization chemistry that was previously developed for formation of DNA microarrays. Preliminary results showed that protein microarrays fabricated on ATMS/diazotized surfaces produced enhanced levels of protein-protein interaction, low background fluorescence and high selectivity. Orientation of the immobilized proteins on the surfaces was also studied. In addition, the antigen-antibody reaction data has been analyzed quantitatively and successfully correlated with solution concentrations. In general, this method allows binding of protein onto a solid substrate that can lead to considerable improvements in antibody-antigen interaction, stability of affixed biomolecules, and preferable protein orientation.