AVS 49th International Symposium
    Biomaterials Wednesday Sessions
       Session BI-WeP

Paper BI-WeP4
Experimental and Modeling Results for Protein Interactions with MEMS Devices

Wednesday, November 6, 2002, 11:00 am, Room Exhibit Hall B2

Session: Biointerfaces and Surfaces II
Presenter: F. Wang, Clemson University
Authors: F. Wang, Clemson University
J. Hickman, Clemson University
R.A. Latour, Clemson University
Correspondent: Click to Email
Microelecromechanical systems (MEMS) for biomedical devices and applications generally are used to interact with simple and complex biological fluids. Undesirable biomolecular surface adsorption, which causes channel plugging, is a leading factor in the failure of such systems. We are attempting to characterize these interactions using a combination of experimental analysis and a computational fluid dynamics (CFD) model. The adsorption process includes transport of biomolecules onto the surface and the adsorptive reaction at the surface. We model the transport by Navier-Stokes equations, which accounts for the mass, momentum and energy conservation for the flow; and the adsorptive reaction is modeled by a modified Langmuir adsorption isotherm. These two parts are integrated by incorporating the surface adsorption kinetics into the transport equation as a boundary condition. A commercially available CFD code, CFD-ACE+, based on a finite volume method is employed as a starting point. BioOpter, a peripheral to this code, has been developed to extract kinetic parameters by minimizing the least-square difference between the simulation and experiment data. We will present preliminary optimization results for experiments in which a 60ng/mL Alkaline Phosphatase solution was passed through a polyethyletherketones(PEEK) modified capillary (65 microns diameter by 100 mm long) at flowrate of 0.1mL/hr which gave the adsorption/desorption parameters as: Ka=7.2 x 10@super 5@/m-s and Kd=2.1@super -5@/s. An optimization error surface model was used to visualize the location of the optimal parameters.