AVS 49th International Symposium
    Biomaterials Wednesday Sessions
       Session BI-WeP

Paper BI-WeP21
Study of Bone Repairing Employing a Ricinus Camunis-based Biopolymer Additioned with Ascorbic Acid and Epidermal Growth Factor in a Rat Tibia Model@footnote 1@

Wednesday, November 6, 2002, 11:00 am, Room Exhibit Hall B2

Session: Biointerfaces and Surfaces II
Presenter: M. Melendez-Lira, Cinvestav-IPN, Mexico
Authors: C. Mendoza-Barrera, UPIITA-IPN and Cinvestav-IPN, Mexico
M. Melendez-Lira, Cinvestav-IPN, Mexico
V. Altuzar, Cinvestav-IPN, Mexico
S.A. Tomas, Cinvestav-IPN, Mexico
Correspondent: Click to Email
There is a huge demand of graft material to accelerate the bone healing process of lesions experienced after surgical interventions related with bony tumors or traumatic experiences. Autografts, allografts and biomaterials are sources of graft materials but it would be highly desirable to have materials without the limitations of the first two aforementioned. We discuss the effect of a Ricinus Camunis-based biopolymer mixed with Ascorbic Acid (AA)and/or Epidermal Growth Factor (EGF) on the bone repairing employing a rat tibia model. The bone healing process was monitored through the interface bone-graft material. No implant rejection or inflammatory reaction was observed during a 8 weeks period in our in vivo studies. The evolution of the osteogenesis in the lesion area was followed employing scanning electron microscopy (SEM), energy dispersive x-ray analysis (EDX), Photoacoustic Spectroscopy (PAS) and X-ray diffraction (XRD). Our study clearly indicates that the combined use of the Ricinus Camunis-based biopolymer, AA and EGF improves the remodeling characteristics of the biomaterial. XRD allow us to identify the structural characteristics of the biopolymer and its evolution during the bone healing process. Bone resorption was monitored by EDX studying the Ca/P ratio as function of time. PAS presents features that could be correlated with cellular activity during the bone mineralization process. All the results showed a good correlation and allowed us to obtain information to improve the composition of the biomaterial. @FootnoteText@ @footnote 1@This work was partially supported by CONACYT-Mexico.