AVS 49th International Symposium
    Biomaterials Wednesday Sessions
       Session BI-WeP

Paper BI-WeP16
Formation of Biotin-reactive Silane Surfaces on SiO@sub 2@ for Specific Immobilization of Biomolecules

Wednesday, November 6, 2002, 11:00 am, Room Exhibit Hall B2

Session: Biointerfaces and Surfaces II
Presenter: H.H.J. Persson, Zyomyx, Inc.
Authors: H.H.J. Persson, Zyomyx, Inc.
L.A. Ruiz-Taylor, Zyomyx, Inc.
D.A. Quincy, Zyomyx, Inc.
S. Follonier, Zyomyx, Inc.
J.K.C. Huang, Zyomyx, Inc.
T.L. Martin, Zyomyx, Inc.
A. Acharya, Zyomyx, Inc.
G. Kilcher, Zyomyx, Inc.
K. Belghiti, Zyomyx, Inc.
H.B. Lu, Zyomyx, Inc.
R.L. Cicero, Zyomyx, Inc.
P. Kernen, Zyomyx, Inc.
P. Wagner, Zyomyx, Inc.
Correspondent: Click to Email
To provide robust and versatile surfaces for protein biochip applications, we have synthesized triethoxy silanes with thiol-reactive groups and report here on the formation of thin films on silicon oxide surfaces. Several surface analytical techniques such as contact angle, XPS, and ellipsometer have been applied to characterize such silane layers. Emphasis was given to layer thickness, wettability, molecular organization, homogeneity and reactivity. Effects of substrate pre-treatment, silanization conditions as well as post-silanization procedures have been evaluated. We used thiol reactive silane layers as reactive intermediates to produce reactive surfaces presenting biotin by in-situ coupling of PEGylated thiol-biotin crosslinkers. Streptavidin has been shown to bind to the surface specifically. Surface coverages, homogeneity, and reactivity of the streptavidin layer were evaluated mainly by radiometry.