AVS 49th International Symposium
    Biomaterials Wednesday Sessions
       Session BI-WeP

Paper BI-WeP15
Nanoporous Aluminum Oxide as Support Material for Enzyme Biosensors

Wednesday, November 6, 2002, 11:00 am, Room Exhibit Hall B2

Session: Biointerfaces and Surfaces II
Presenter: A. Heilmann, Fraunhofer-Institute for Mechanics of Materials, Germany
Authors: A. Heilmann, Fraunhofer-Institute for Mechanics of Materials, Germany
N. Teuscher, Fraunhofer-Institute for Mechanics of Materials, Germany
D. Janasek, Martin-Luther-University, Germany
U. Spohn, Martin-Luther-University, Germany
Correspondent: Click to Email
Enzyme sensors are of growing interest as detection tool in various clinical and food analysis. Up to now, limited operational stability of enzyme is even yet the main hindrance to their wider application to solve analytical problems. In the paper, we describe a novel method to create biosensors with good long-time stability by using nanoporous alumina oxide with well-defined pore sturcture as host material for enzyme immobilisation. The nanoporous aluminum oxide was made by anodic oxidation od alumnium in polyprotic acids. Free-standig membranes were realized by lifting the membrane film from the metal substrate. In this free-standig membranes, different enzymes were immobilized. e.g. pyruvate oxidase (PyrOD) from lactobacillus plantarum was enclosed in poly(carbamoylsulphonate) hydrogel and sucked into the porous structure before polymerization. In the paper, calibration curves and long-time stability of varous enzyme sensors were disccused, also by consideration the nanostructure of the filled alumina pores studied by scanning electron microscopy.