AVS 49th International Symposium
    Biomaterials Wednesday Sessions
       Session BI-WeP

Paper BI-WeP12
X-Ray PhotoEmission Electron Microscopy of Micro-contact Printed Protein and Polymer Coated Surfaces

Wednesday, November 6, 2002, 11:00 am, Room Exhibit Hall B2

Session: Biointerfaces and Surfaces II
Presenter: C. Morin, McMaster University, Canada
Authors: C. Morin, McMaster University, Canada
A.P. Hitchcock, McMaster University, Canada
D.G. Castner, University of Washington
B. Wickes, University of Washington
A. Scholl, Lawrence Berkeley National Laboratory
A. Doran, Lawrence Berkeley National Laboratory
Correspondent: Click to Email
Synthetic biomaterials are widely used in medical applications. However, their interaction with the body is mediated through passive adsorption of a disorganized adsorbed protein monolayer. Mis-recognition of this adsorbed disorganized protein layer by surrounding cells may lead to the classic foreign body reaction and device encapsulation.@footnote 1@ Next generation biomaterials, or 'engineered biomaterials', are being designed in which the surface contains specific bio-recognition moieties which control the biological response of the host. Microcontact printing (µCP) is one such method which can deposit biological signalling agents with spatial resolution and fidelity.@footnote 2@ It uses an elastomeric template to transfer protein molecules to a surface of interest. µCP is combined with thiol-Au self assembly to form patterns on surfaces. We are exploring the use of X-Ray photoemission electron microscopy@footnote 3@ to monitor methods for preparing patterned functionalized biomaterial surfaces, and to investigate the specificity of the interaction of model surfaces with key proteins. To test the reliability of the surface patterning method, we use highly specific bio-recognition pairs, such as the biotinylated ferritin-streptavidin couple, to probe the quality of the patterned surface. Such structures are then investigated with elemental (Fe 2p) and molecular (C1s and N1s) speciation using NEXAFS microscopy recorded at the ALS BL 7.3.1 X-PEEM. This work is supported by research and partnership grants from NSERC (Canada) and a Canada Research Chair (APH). NESAC/BIO (DGC) is supported by NIH grant RR-01296 from the National Center for Research Resources. ALS is supported by U.S. DoE under contract DE-FG02-89ER60858. @FootnoteText@ @footnote 1@ Ratner, B.D., J. Molecular Recognition, 9 (1996) 617. @footnote 2@ Zhao, X.-M., Y. Xia, and G.M. Whitesides, J. Materials Chemistry 7 (1997) 1069@footnote 3@ S. Anders, et al. Rev. Sci. Inst. 70 (1999) 3973.