AVS 49th International Symposium
    Biomaterials Wednesday Sessions
       Session BI-WeA

Paper BI-WeA4
Cellular Interactions with Multilayered Polyelectrolyte Films

Wednesday, November 6, 2002, 3:00 pm, Room C-201

Session: Polyelectrolyte Surfaces/Cell-Surface Interactions
Presenter: J.C. Voegel, INSERM Unite 424, France
Authors: C. Picart, Université Louis Pasteur, France
Ph. Lavalle, INSERM Unite 424, France
L. Richert, Université Louis Pasteur, France
D. Vautier, Université Louis Pasteur, France
P. Schaaf, CNRS, France
J.C. Voegel, INSERM Unite 424, France
Correspondent: Click to Email
The short time interactions of chondrosarcomas cells with polyelectrolyte multilayered architectures built up by the alternated deposition of poly(L-lysine) (PLL) and poly(L-glutamic acid) (PGA) were estimated in the presence and the absence of serum. Film constructions with and without protein adsorption were first characterized by means of optical waweguide lightmode spectroscopy, quartz cristal microbalance and zeta potential determinations. In the presence of a serum containing medium, the detachment forces measured by the micropipette technique were about eight times smaller for PGA than for PLL ending films. For these later ones, the adhesion forces decreased also when the films increased in thickness. In a serum-free medium the differences between the negative and positive ending films become larger : adhesion forces on PLL-ending films were by 40 % to 100 % higher, whereas no cells adhered on PGA terminating films. Also, PGA ending films were found to prevent the adsorption of serum proteins whereas large protein amounts adsorbed always on PLL ending films. These data suggest that cell interactions with polyelectrolyte films can be tuned by the type of the outermost layer, by the presence of proteins, and the number of deposited layers.