AVS 49th International Symposium
    Biomaterials Wednesday Sessions
       Session BI-WeA

Paper BI-WeA3
Poly-(L-Glutamic Acid)/Poly-(L-lysine) Multilayers used as Biomaterial Coating

Wednesday, November 6, 2002, 2:40 pm, Room C-201

Session: Polyelectrolyte Surfaces/Cell-Surface Interactions
Presenter: P. Schaaf, Institut Charles Sadron (CNRS), France
Authors: P. Schaaf, Institut Charles Sadron (CNRS), France
J.C. Voegel, Unite INSERM U424, France
C. Picart, Unite INSERM U424, France
Ph. Lavalle, Unite INSERM U424, France
F. Boulmedais, Institut Charles Sadron (CNRS), France
Correspondent: Click to Email
Polyelectrolyte multilayers constitute an easy tool for multi-functionalizing biomaterial surfaces. In this paper we will discuss biological and physico-chemical properties of poly-(L-glutamic acid)/poly-(L-lysine) (PGA/PLL) multilayers. We first discuss the response of cells in contact with (PGA/PLL) multilayers functionalized by embedding active peptides within the films. We then present results relative to the cellular response when the film is functionalized by embedding proteins in the architecture. In both cases we get a cellular response even when the active molecules are embedded under up the 20 (PGA/PLL) bilayers. We discuss also possible cell recognition mechanisms of the active molecules. But the system (PGA/PLL) is also interesting because it leads to films with internal secondary structures such as beta-sheets or alpha-helices. The relation between the secondary structure of the film and the secondary structure of (PLL/PGA) complexes in solution will also be presented.