AVS 49th International Symposium
    Biomaterials Tuesday Sessions
       Session BI+SS-TuM

Paper BI+SS-TuM9
Surface Characterisation of Supported Lipid Layers

Tuesday, November 5, 2002, 11:00 am, Room C-201

Session: Platforms for Non-fouling and Patterned Surfaces
Presenter: S.L. McArthur, University of Washington
Authors: S.L. McArthur, University of Washington
M.W. Halter, University of Washington
V. Vogel, University of Washington
D.G. Castner, University of Washington
Correspondent: Click to Email
The boundaries of biological cells and organelles are defined by complex and dynamic membranes constructed from an array of lipids, proteins and carbohydrates. These interfaces have a range of specific functions and properties, one of which is their ability to prevent non-specific protein adsorption, making membrane mimics an attractive option for a variety of in vivo and in vitro biomedical implant and diagnostic applications. The development and characterization of complex biomimetic surfaces presents a challenge in terms of their initial formation, long-term stability and integrity in a variety of environments and the maintenance of bilayer fluidity. In this study we detail the development and chemical characterization of supported lipid monolayers. The structure was formed by coupling HEMA to a glass support and subsequently activating it with CDI to couple the headgroups of the lipid, dimyristoyl ethanolamine (DMPE). The success of the immobilization procedure was investigated by XPS and ToF-SIMS. A number of different lipid transfer regimes were explored. Results illustrated that the samples produced using Langmuir-Blodgett transfer at high pressure (20 mN/m) had the largest fraction of the transferred lipids remaining at the surface after 5 minutes sonication in ethanol. Fluorescence microscopy of the lipid layers showed that the presence of this limited number of anchored lipids acted to stabilize the monolayer and maintain its integrity without having a detrimental effect on layer fluidity.