AVS 49th International Symposium
    Biomaterials Wednesday Sessions
       Session BI+AS-WeM

Paper BI+AS-WeM4
Investigation of Protein Adsorption with Simultaneous Measurements of Atomic Force Microscope (AFM) and Quartz Crystal Microbalance (QCM)

Wednesday, November 6, 2002, 9:20 am, Room C-201

Session: Ambient Surface Science Techniques
Presenter: K.-H. Choi, IMEC, Belgium
Authors: K.-H. Choi, IMEC, Belgium
J.-M. Friedt, IMEC, Belgium
F. Frederix, IMEC, Belgium
W. Laureyn, IMEC, Belgium
A. Campitelli, IMEC, Belgium
G. Borghs, IMEC, Belgium
Correspondent: Click to Email
We have combined the tapping mode atomic force microscope (AFM) and quartz crystal microbalance (QCM) for the direct investigation and characterization of protein adsorption on various metallic surfaces. The adsorption of proteins, such as human plasma fibrinogen, @gamma@-globulin and collagen, onto the metal/QCM surface were monitored using both methods at the same time when varying the concentration of them. We present the AFM images that shows the surface changes and the adsorption scheme of proteins with molecular resolution according to the shift of resonant vibration frequency of the QCM. The combination of AFM with QCM and the simultaneous measurements of the bio molecule adsorption with two techniques provide us with not only the sensing and detection technique but also the means for understanding the adsorption schemes of bio molecules on the metal surface.