IUVSTA 15th International Vacuum Congress (IVC-15), AVS 48th International Symposium (AVS-48), 11th International Conference on Solid Surfaces (ICSS-11)
    Thin Films Thursday Sessions
       Session TF+BI-ThM

Invited Paper TF+BI-ThM3
Desorption and Processing of Bioactive Thin Films

Thursday, November 1, 2001, 9:00 am, Room 123

Session: Bioactive and Organic/Inorganic Thin Films
Presenter: A. Chilkoti, Duke University
Correspondent: Click to Email
I will describe methods to micro- and nano-pattern proteins and other biological ligands onto self-assembled monolayers (SAMs) and polymers for application in multianalyte biosensors, patterned biomaterials, and protein chips. These methods include: (1) Light-activated micropatterning (LAMP), which exploits spatially precise, light-activated deprotection of affinity ligands on functionalized SAMs to achieve step-and-repeat patterning of multiple biomolecules. (2) Microstamping onto activated polymer surfaces (MAPS), which involves surface-selective functionalization of polymers, followed by microcontact printing of reactive biological ligands. (3) Thermodynamically addressable reversible patterning (TRAP) which uses patterned domains with different surface energies as a thermodynamic address to direct the attachment of proteins and other biomolecules from solution. TRAP functions by the selective adsorption of nanoclusters of an elastin fusion protein above its phase transition temperature specifically on patterned hydrophobic regions, but not on a hydrophilic background. Unlike other methods for protein patterning, TRAP is reversible, and modulating the solution environment (e.g., T, ionic strength), can erase protein patterns. A theme illustrated by this talk will be the interdisciplinary convergence of surface chemistry and spectroscopic characterization (XPS, TOF-SIMS, and evanescent optical techniques) with molecular biology.