IUVSTA 15th International Vacuum Congress (IVC-15), AVS 48th International Symposium (AVS-48), 11th International Conference on Solid Surfaces (ICSS-11)
    Surface Science Monday Sessions
       Session SS1-MoA

Paper SS1-MoA7
Desorption/Ionization on Porous Silicon (DIOS) Mass Spectrometry: A New Methodology for Bioanalysis

Monday, October 29, 2001, 4:00 pm, Room 120

Session: Innovations in Surface Science
Presenter: Z. Shen, University of California, San Diego
Authors: Z. Shen, University of California, San Diego
J.E. Crowell, University of California, San Diego
G. Siuzdak, The Scripps Research Institute
Correspondent: Click to Email
DIOS-MS is a new mass spectrometry strategy based on pulsed laser desorption/ionization from a porous silicon surface. DIOS-MS is similar to matrix-assisted laser-desorption ionization mass spectrometry (MALDI-MS) in that it utilizes the same instrument; however, in DIOS-MS, porous silicon is used to trap analytes deposited on the surface and laser radiation is used to vaporize and ionize these molecules, without the presence of any matrix material. We have shown that DIOS-MS can be used for a wide range of small molecules as well as biomolecules at the femtomole and attomole level with little or no fragmentation, in contrast to what is typically observed with other direct desorption/ionization approaches. DIOS-MS offers many unique advantages including good sensitivity, low background ion interference, and high salt tolerance. We will demonstrate the application of DIOS-MS to small molecule quantitative analysis, chemical reaction monitoring, enzyme-substrate reaction and inhibition characterization, and drug metabolism study. We will also demonstrate DIOS-MS as a unique scanning approach for small-molecule high throughput analysis.