IUVSTA 15th International Vacuum Congress (IVC-15), AVS 48th International Symposium (AVS-48), 11th International Conference on Solid Surfaces (ICSS-11)
    Biomaterials Tuesday Sessions
       Session BI-TuP

Paper BI-TuP9
Morphological Analysis of the Collagen Structure of Regenerated Rat Tendons Following Laser Photo-stimulation

Tuesday, October 30, 2001, 5:30 pm, Room 134/135

Session: Surface Characterization and Non-Fouling Surfaces Poster Session
Presenter: V. Baranauskas, Universidade de Campinas - Brazil
Authors: V. Baranauskas, Universidade de Campinas - Brazil
N.V. Parizotto, Universidade Federal de Sao Carlos - Brazil
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Atomic Force Microscopy (AFM) and Scanning Electron Microscopy (SEM) were used to comparatively study the structure of regenerated rat tendons submitted to laser photo-stimulation after a surgical injury that extracted around 6 mm of Achilles'' tendon (tenectomy) of the left forefoot. The experiment was performed using male Rattus norvegicus albinus, lineage Wistar, with body weights in the range of 250 to 300 g, and 90 days old. The healing course was monitored at 7, 14, 21 or 28 days after the injury. The animals were divided into four groups. One group was used as a control and the other three were subjected to irradiation of doses of 0.5, 5.0 and 50 Jcm-2, respectively. A He-Ne laser of 6 mW power (L = 632.8 nm) was used. AFM and SEM images, at different magnifications, allowed the identification of the time-dependence of the recovery, measured by the organization of the collagen fibers. Comparison of natural recovery processes with the laser photo-stimulation procedures will be discussed.