IUVSTA 15th International Vacuum Congress (IVC-15), AVS 48th International Symposium (AVS-48), 11th International Conference on Solid Surfaces (ICSS-11)
    Biomaterials Tuesday Sessions
       Session BI-TuP

Paper BI-TuP3
Fast Impedance Spectroscopy Measurements on Supported Lipid Bilayer Membranes with and without Incorporated Ion Channels

Tuesday, October 30, 2001, 5:30 pm, Room 134/135

Session: Surface Characterization and Non-Fouling Surfaces Poster Session
Presenter: G. Wiegand, Zyomyx Inc.
Authors: G. Wiegand, Zyomyx Inc.
S. Beyer, Zyomyx Inc.
N. Arribas-Layton, Zyomyx Inc.
P. Wagner, Zyomyx Inc.
Correspondent: Click to Email
A substantial part of the mammalian proteome is represented by proteins that are either associated or incorporated into lipid bilayer membranes. Our goal is to provide appropriate platform assays and transducer technologies for the functional analysis of membrane proteins. Our special focus is on ion channels due to their pharmacological relevance. Because ionic flux thru an ion channel generates an electrical signal, electronic transducer technologies are the most direct detection method for ion channel analysis. We developed a method of fast impedance spectroscopy that combines the power of a spectroscopic technique providing high information content with the millisecond time resolution of a fast analytical tool. In biophysical experiments, time dependent quantities such as the membrane resistance and the membrane capacity are obtained from the measured sequences of impedance spectra. Supported lipid bilayers provide membrane matrices for protein incorporation that are coupled to solid surfaces. Supported bilayer applications take advantage of the high membrane stability imparted by the solid support, and of the improved accessibility for analytical tools due to the two-dimensional geometry. As a result of the chip compatibility, supported membrane systems are potentially useful in high-throughput technologies. By application of fast impedance spectroscopy, dynamic properties of supported lipid bilayers with and without incorporated ion channels are studied during formation, relaxation and in various states of conduction.