IUVSTA 15th International Vacuum Congress (IVC-15), AVS 48th International Symposium (AVS-48), 11th International Conference on Solid Surfaces (ICSS-11)
    Biomaterials Tuesday Sessions
       Session BI-TuP

Paper BI-TuP16
Oligo(Ethylene Glycol)-Terminated Self Assembled Monolayers: Protein Resistance and the Effect of Assembly Temperature

Tuesday, October 30, 2001, 5:30 pm, Room 134/135

Session: Surface Characterization and Non-Fouling Surfaces Poster Session
Presenter: C. Boozer, University of Washington
Authors: C. Boozer, University of Washington
S. Chen, University of Washington
L. Li, University of Washington
S. Jiang, University of Washington
Correspondent: Click to Email
The rational design of protein resistant surfaces is a critical step in the ongoing development of biomaterials and biosensors, yet we lack a fundamental understanding of how such surfaces work. Here, we report a systematic study of the behavior of oligo(ethylene glycol)-terminated self-assembled monolayers (SAMs) prepared at a range of temperatures. The monolayers were formed by self-assembly of (EG)6-terminated thiols, in a heated (or cooled) methanol solution, on both single crystal and polycrystalline gold films. The films were characterized using atomic force microscopy (AFM), X-ray photoelectron spectroscopy (XPS), and infrared adsorption (FTIR). Protein adsorption on the OEG-terminated SAMs was studied using a home-built surface plasmon resonance (SPR) sensor. It was found that the ability of the OEG-terminated SAMs to resist protein adsorption from a buffer solution correlates with the temperature at which they were prepared. Protein adsorption studies were performed with both bovine serum albumin and fibrinogen, and in both cases we found that protein resistance of the films was greatly diminished by increasing assembly temperature. A possible mechanism will be presented.