IUVSTA 15th International Vacuum Congress (IVC-15), AVS 48th International Symposium (AVS-48), 11th International Conference on Solid Surfaces (ICSS-11)
    Biomaterials Thursday Sessions
       Session BI-ThP

Paper BI-ThP7
Dynamics and Chemistry of ELISA Test for Toxins by In-situ and Ex-situ Spectroscopic Ellipsometry

Thursday, November 1, 2001, 5:30 pm, Room 134/135

Session: Biomolecule and Cell Poster Session
Presenter: D.W. Thompson, University of Nebraska-Lincoln
Authors: D.W. Thompson, University of Nebraska-Lincoln
E.M. Berberov, University of Nebraska-Lincoln
G.L. Pfeiffer, University of Nebraska-Lincoln
J.A. Woollam, University of Nebraska-Lincoln
T.E. Tiwald, J.A. Woollam Co., Inc.
C.M. Herzinger, J.A. Woollam Co., Inc.
T. Datta, University of South Carolina
Correspondent: Click to Email
Fast, in-situ spectroscopic ellipsometry at 225 simultaneous visible wavelengths is used to study dynamics of deposition in liquid solution of each layer in a four-layer enzyme-linked immunosorbent assay (ELISA) for cholera toxin. Time-dependent functional relationships of cholera and Escherichia coli heat-labile enterotoxin attachment to monosialoganglioside (G@subM1@) coated substrates are studied, as well as the attachment of associated antibodies. Ellipsometric selectivity between toxins is demonstrated. In addition, ex-situ spectroscopic ellipsometry from vacuum-ultraviolet (131 nm / 9.5 eV) to mid-infrared (35 microns) on each constituent layer of the ELISA is studied. Several distinct oscillator-like features in the visible to vacuum-UV are found at 1.42 eV, 4.24 eV, 6.32-6.35 eV, and 10.98 eV. Protein resonant chemical oscillators in the individual layers are identified as: C-H in CH@sub2@ and CH@sub3@, N-H, O-H, and P=O, seen at 3307 cm@super-1@, 2926 cm@super-1@, 2958 cm@super-1@, 1662 cm@super-1@, 1546 cm@super-1@, 1245 cm@super-1@ and 1081 cm@super-1@. In summary, in-situ and ex-situ spectroscopic ellipsometry covering vacuum-UV to mid-infrared is a simple, nondestructive way to study dynamics and chemistry of nanometer dimension ELISA films. Research supported by NSF SBIR contract number NSF II-9901510.