IUVSTA 15th International Vacuum Congress (IVC-15), AVS 48th International Symposium (AVS-48), 11th International Conference on Solid Surfaces (ICSS-11)
    Biomaterials Monday Sessions
       Session BI-MoP

Paper BI-MoP8
Measuring Bound Water in Protein-resistant Coatings: A Combined OWLS and QCM-D Study of Poly(L-lysine)-g-poly(ethylene glycol)

Monday, October 29, 2001, 5:30 pm, Room 134/135

Session: Biorecognition Poster Session
Presenter: S.M. De Paul, ETH Zürich, Switzerland
Authors: S.M. De Paul, ETH Zürich, Switzerland
J. Vörös, ETH Zürich, Switzerland
I. Reviakine, ETH Zürich, Switzerland
C. Galli, University of Fribourg, Switzerland
M. Collaud Coen, University of Fribourg, Switzerland
M. Textor, ETH Zürich, Switzerland
N.D. Spencer, ETH Zürich, Switzerland
Correspondent: Click to Email
Metal oxide surfaces coated with poly(L-lysine)-g-poly(ethylene glycol) (PLL-g-PEG) have been shown to resist non-specific adsorption of proteins. The hydrophilicity of the PEG chains is believed to play a crucial role in such behavior. In order to determine the amount of water coupled to PLL-g-PEG at aqueous metal oxide interfaces, we use results from two in situ methods: optical waveguide lightmode spectroscopy (OWLS), which detects the mass of polymer adsorbed at the surface, and quartz crystal microbalance with dissipation (QCM-D), which is sensitive to the mass of the polymer plus the mass of trapped or hydrodynamically coupled water. Complementary information about water content is provided by solid-state NMR measurements. We also examine how the amount of adsorbed polymer and its protein resistance vary with the choice of substrate (e.g., TiO@sub 2@, SiO@sub 2@) and with the surface topography as determined by atomic force microscopy (AFM).