IUVSTA 15th International Vacuum Congress (IVC-15), AVS 48th International Symposium (AVS-48), 11th International Conference on Solid Surfaces (ICSS-11)
    Biomaterials Monday Sessions
       Session BI-MoP

Paper BI-MoP7
Reactivities and Biomolecular Immobilization on Self-assembled Alkanethiols with @OMEGA@-substituted N-hydroxysuccinimide-ester Groups on Au(111)

Monday, October 29, 2001, 5:30 pm, Room 134/135

Session: Biorecognition Poster Session
Presenter: S. McManus-Munoz, Zyomyx, Inc.
Authors: S. McManus-Munoz, Zyomyx, Inc.
D. Martin, Zyomyx, Inc.
C.E.J. Dentinger, Zyomyx, Inc.
R.L. Cicero, Zyomyx, Inc.
H. Tran, Zyomyx, Inc.
M. Chen, Zyomyx, Inc.
P. Kernen, Zyomyx, Inc.
P. Wagner, Zyomyx, Inc.
Correspondent: Click to Email
Alkanethiols with @omega@-substituted N-hydroxysuccinimide ester groups have been self-assembled on Au(111) surfaces. Stability and reactivity of the functional group were studied using reflection absorption infrared spectroscopy, contact angle, ellipsometry and radiometry measurements. Increased NHS-reactivity was observed for alkanethiols containing (oligo)ethylene glycol units. Effective NHS quenching combined with some effects on non-specific protein adsorption was found with various amino-containing compounds, e.g. glycine and amino-PEGs. Transformation of the NHS-group into a biotin exposing surface was obtained by in-situ coupling of amino-biotin. Specificity and homogeneity of streptavidin and biotin-conjugated protein binding was tested by spectroscopic and microscopic techniques and compared with the binding efficiencies on physisorbed polymer layers of biotin derivatized poly(L-lysine)-grafted poly(ethylene glycol) on metal oxides and on Au(111).