IUVSTA 15th International Vacuum Congress (IVC-15), AVS 48th International Symposium (AVS-48), 11th International Conference on Solid Surfaces (ICSS-11)
    Biomaterials Monday Sessions
       Session BI-MoP

Paper BI-MoP6
Biotin-reactive Surfaces Based on @OMEGA@-substituted Alkanethiols on Au(111)

Monday, October 29, 2001, 5:30 pm, Room 134/135

Session: Biorecognition Poster Session
Presenter: H. Tran, Zyomyx, Inc.
Authors: H. Tran, Zyomyx, Inc.
M. Chen, Zyomyx, Inc.
H. Lu, Zyomyx, Inc.
A. Neurauter, Zyomyx, Inc.
S. McManus-Munoz, Zyomyx, Inc.
D. Quincy, Zyomyx, Inc.
T. Langenbacher, Zyomyx, Inc.
P. Peluso, Zyomyx, Inc.
P. Kernen, Zyomyx, Inc.
S. Nock, Zyomyx, Inc.
P. Wagner, Zyomyx, Inc.
Correspondent: Click to Email
(Oligo)ethylene glycol-containing alkanethiols with @omega@-substituted N-hydroxysuccinimide-ester groups self-assemble in ordered monolayers on Au(111) surfaces (NHS-SAM) and form highly reproducible reactive interfaces for in-situ transformations and biomolecular immobilization. Surface sensitive spectroscopic techniques including FTIRRAS, and XPS, indicate that amino-biotin can be successfully coupled in-situ to the NHS-SAM. Such reactive surfaces have been tested for homogeneous surface coverage and selective binding of streptavidin- and biotin-conjugated dyes and proteins using fluorimetry, radiometry and surface plasmon resonance. These binding data on biotin-functionalized SAMs are compared with binding efficiencies of electrostatically adsorbed biotin derivatized poly(L-lysine)-grafted poly(ethylene glycol) layers on Au(111) surfaces. We incorporated these bioreactive interfaces into microfabricated three-dimensional structures in silicon and used these to test an immunoassay in a microarray format.