IUVSTA 15th International Vacuum Congress (IVC-15), AVS 48th International Symposium (AVS-48), 11th International Conference on Solid Surfaces (ICSS-11)
    Biomaterials Monday Sessions
       Session BI-MoP

Paper BI-MoP4
Organization of Multifunctional Co-polymers on Metal Oxide Surfaces for Optical Biosensing Applications

Monday, October 29, 2001, 5:30 pm, Room 134/135

Session: Biorecognition Poster Session
Presenter: I. Reviakine, ETH Zürich, Switzerland
Authors: N.-P. Huang, ETH Zürich, Switzerland
I. Reviakine, ETH Zürich, Switzerland
S.M. De Paul, ETH Zürich, Switzerland
M. Textor, ETH Zürich, Switzerland
N.D. Spencer, ETH Zürich, Switzerland
Correspondent: Click to Email
A novel polymeric interface that combines the resistance to non-specific protein adsorption conferred to metal oxide surfaces by poly(L-lysine)-g-poly(ethylene glycol) (PLL-g-PEG) with the high affinity of the well-known (strept)avidin-biotin system through the use of a biotinylated-PEG-containing copolymer (PLL-g-PEG/PEGbiotin) has recently been introduced by our group for use in biosensing applications. Biosensor chips with high sensitivity and selectivity can be designed with the above approach, making it an attractive surface technology. Optimization of this system for various biosensor applications depends critically on the knowledge of how the polymer film is organized at the metal oxide interface. Therefore, this work focuses on investigating the organization of the co-polymer film by atomic force microscopy (AFM), quartz crystal microbalance with dissipation (QCM-D) and optical waveguide lightmode spectroscopy (OWLS). This combination of spectroscopic and imaging techniques provides an insight into how the co-polymer mixture is organized at the surface and how this organization evolves with time. The effect of streptavidin addition was also investigated and biotinylated liposomes were used as a well-understood model analyte in AFM and QCM studies.