IUVSTA 15th International Vacuum Congress (IVC-15), AVS 48th International Symposium (AVS-48), 11th International Conference on Solid Surfaces (ICSS-11)
    Biomaterials Friday Sessions
       Session BI-FrM

Paper BI-FrM6
Electrostatically Assisted SPR: A New Method for DNA Mutation Detection

Friday, November 2, 2001, 10:00 am, Room 102

Session: Biosensors
Presenter: L.K. Wolf, Boston University
Authors: L.K. Wolf, Boston University
R.J. Heaton, Boston University
A.W. Peterson, Boston University
R.M. Georgiadis, Boston University
Correspondent: Click to Email
Detection of DNA base-pair mutations is essential for the study and treatment of genetic disorders and disease. Present-day studies use DNA microarrays and fluorescently labeled probes for high-throughput mutation analysis. Recently, we have discriminated between matched and mismatched surface immobilized duplexes by evaluating surface coverage and hybridization kinetics with electrostatically assisted surface plasmon resonance (SPR).@footnote 1@ Here, we show that this method allows label-free in-situ detection of a single A-C base-pair mismatch in a 25-mer oligonucleotide duplex. We also apply electrostatically assisted SPR to other types of mismatches, demonstrating the generality and usefulness of this method to mutation detection. The effects of ionic strength on mismatch discrimination are also investigated. @FootnoteText@ @footnote 1@ R. J. Heaton, A. W. Peterson, and R. M. Georgiadis, Proc. Nat. Acad. Sci. 98, 3701 (2001).