IUVSTA 15th International Vacuum Congress (IVC-15), AVS 48th International Symposium (AVS-48), 11th International Conference on Solid Surfaces (ICSS-11)
    Biomaterials Friday Sessions
       Session BI-FrM

Paper BI-FrM5
Comparative Analysis of Immobilization Strategies for Antibodies and Their Fragments on Biosensor Surfaces

Friday, November 2, 2001, 9:40 am, Room 102

Session: Biosensors
Presenter: P. Peluso, Zyomyx, Inc.
Authors: P. Peluso, Zyomyx, Inc.
D. Wilson, Zyomyx, Inc.
D. Do, Zyomyx, Inc.
M. Venkatasubbaiah, Zyomyx, Inc.
H. Tran, Zyomyx, Inc.
P. Kernen, Zyomyx, Inc.
K. Witte, Zyomyx, Inc.
B. Heidecker, Zyomyx, Inc.
P. Wagner, Zyomyx, Inc.
S. Nock, Zyomyx, Inc.
Correspondent: Click to Email
Advancement in the study of proteomics requires the development of microarray biosensors which possess a high degree of sensitivity. Towards this goal, strategies must be optimized to couple biomolecular capture agents to matrices at sufficient densities without compromising their binding properties. Using SPR and radioimmunoassay methods, we have examined the effect of different coupling chemistries on the binding activities of various antibodies and their corresponding Fab forms. Novel methods for attaching antibodies and Fab fragments to surfaces were employed and compared with random coupling procedures. In general, we found oriented attachment to better maintain the binding activity of these molecules. Moreover, oriented-Fab surfaces appear to exhibit the highest molecular density and binding activity. We further extrapolated these findings to performance within a microarray format utilizing a fluorescence-based sandwich assay.