IUVSTA 15th International Vacuum Congress (IVC-15), AVS 48th International Symposium (AVS-48), 11th International Conference on Solid Surfaces (ICSS-11)
    Biomaterials Wednesday Sessions
       Session BI+SS-WeM

Paper BI+SS-WeM8
Control and Detection of Surface Immobilized DNA Interactions Using Electrostatically Assisted Surface Plasmon Resonance

Wednesday, October 31, 2001, 10:40 am, Room 102

Session: Biological Interface & Surface Science
Presenter: R.J. Heaton, Boston University
Authors: R.J. Heaton, Boston University
A.W. Peterson, Boston University
L.K. Wolf, Boston University
R.M. Georgiadis, Boston University
Correspondent: Click to Email
Using Surface Plasmon Resonance (SPR) spectroscopy, we demonstrate that the formation of an immobilized DNA film on gold can be controlled by non-Faradaic electrostatic charging. Furthermore, electrostatic charging can be used to enhance interactions between the immobilized probe film and target oligonucleotides in solution. By simply adjusting the potential across the surface thiol-modified single stranded oligonucleotide films can be formed with good reproducibility in coverage. The application of an attractive potential can speed up and maximize the hybridization of complementary and mismatched oligonucleotides to the probe film. We demonstrate the efficacy of this technology with application to both single area and array-mode SPR.