IUVSTA 15th International Vacuum Congress (IVC-15), AVS 48th International Symposium (AVS-48), 11th International Conference on Solid Surfaces (ICSS-11)
    Biomaterials Wednesday Sessions
       Session BI+SS-WeM

Paper BI+SS-WeM7
Thiol-Reactive Surfaces for the Specific Immobilization of Biomolecules

Wednesday, October 31, 2001, 10:20 am, Room 102

Session: Biological Interface & Surface Science
Presenter: R.L. Cicero, Zyomyx Inc.
Authors: R.L. Cicero, Zyomyx Inc.
D. Martin, Zyomyx Inc.
S. McManus-Munoz, Zyomyx Inc.
C.E.J. Dentinger, Zyomyx Inc.
P. Kernen, Zyomyx Inc.
P. Wagner, Zyomyx Inc.
Correspondent: Click to Email
We present multi-component self-assembled monolayers on Au(111) surfaces that extend thiol-reactive groups and chemical moieties known to resist non-specific adsorption of proteins. Particular attention will be paid to the structural characterization of these surfaces using X-ray photoelectron spectroscopy, reflection absorption infrared spectroscopy and ellipsometry. We also investigate the effect surface density of thiol-reactive groups presented at the solid-liquid interface has with respect to homogeneity, efficiency and specificity of immobilizing thiol-containing biomolecules to these surfaces. Several methods for detecting and quantifying biomolecule immobilization are used including surface plasmon resonance spectrometry, radiometry and fluorimetry.