IUVSTA 15th International Vacuum Congress (IVC-15), AVS 48th International Symposium (AVS-48), 11th International Conference on Solid Surfaces (ICSS-11)
    Biomaterials Wednesday Sessions
       Session BI+SS-WeM

Paper BI+SS-WeM10
Vesicle to Supported Bilayer Transformation Kinetics; Influence from Vesicle Size, Temperature and Surface Support

Wednesday, October 31, 2001, 11:20 am, Room 102

Session: Biological Interface & Surface Science
Presenter: E. Reimhult, Chalmers University of Technology, Sweden
Authors: E. Reimhult, Chalmers University of Technology, Sweden
K. Dimitrievski, Chalmers University of Technology, Sweden
V.P. Zhdanov, Chalmers University of Technology, Sweden
F. Höök, Chalmers University of Technology, Sweden
B. Kasemo, Chalmers University of Technology, Sweden
Correspondent: Click to Email
Supported phospholipid bilayers (SPB) on solid surfaces are biologically functional components of high current interest, e.g., for biosensors, tissue engineering, and basic science (Sackman, Science 271:43 (1996)). We investigate how the kinetics of vesicle to bilayer transformation on SiO@sub 2@ depend on vesicle size using small Extruded Unilamellar Vesicles (EUV; diameter~30-200 nm) and Small sonicated Unilamellar Vesicles (SUV; diameter~25 nm)) and temperature (T~5 to 30°C). ). The experimental results are complemented by computer modeling and MC simulations. Our results reveal weak but significant vesicle size-dependent kinetics. The rate and completeness of the vesicle-to-bilayer transformation is strongly dependent on temperature and the vesicle-to-bilayer formation on SiO@sub 2@ can under certain circumstances be completely inhibited at low temperatures. In addition, the vesicle-surface interaction was investigated for various surfaces, including oxidized Au, Pt and Ti, which all demonstrate adsorption of vesicles in an intact state independent of vesicle size and temperature. The obtained results extend our previous studies at constant vesicle size and temperature (Keller et al, Phys Rev B 61: (3) 2291 (2000)) and constitute a platform that will significantly improve the possibility to control the process on µmm-nm fabricated surfaces, from which more complex functional supported bio-membranes are constructed.