IUVSTA 15th International Vacuum Congress (IVC-15), AVS 48th International Symposium (AVS-48), 11th International Conference on Solid Surfaces (ICSS-11)
    Biomaterials Wednesday Sessions
       Session BI+AS-WeA

Paper BI+AS-WeA3
Quantitative Analysis of Multicomponent Adsorbed Protein Films by Static Time of Flight Secondary Ion Mass Spectrometry

Wednesday, October 31, 2001, 2:40 pm, Room 102

Session: Surface Characterization
Presenter: M.S. Wagner, University of Washington
Authors: M.S. Wagner, University of Washington
M. Shen, University of Washington
T.A. Horbett, University of Washington
D.G. Castner, University of Washington
Correspondent: Click to Email
Quantitative analysis of multicomponent adsorbed protein films is an integral part in the investigation of biofouling in many marine, food processing, and biomaterial applications. We have previously shown that Time of Flight Secondary Ion Mass Spectrometry (ToF-SIMS) and multivariate analysis (MVA) are ideal techniques for the analysis of single component adsorbed protein films.@footnote 1@ MVA is also essential for quantitative compositional analysis of multicomponent adsorbed protein films in one parallel ToF-SIMS experiment. Partial Least Squares Regression (PLSR), a multivariate calibration technique, can quantitatively determine the composition of binary protein mixtures adsorbed onto mica using only the single component spectra for calibration. Current research focuses on binary protein mixtures on fluorocarbon and nitrogen-containing plasma polymer surfaces, both providing unique challenges for quantitative analysis by ToF-SIMS. Ternary and quaternary adsorbed protein films adsorbed onto mica were also investigated to determine the limit of complexity for quantitative compositional analysis. Finally, Principal Component Analysis (PCA) and a set of standard spectra were used to obtain semi-quantitative compositional analysis of a time series protein adsorption from dilute plasma solutions. Factor-deficient (fibrinogen and kininogen) plasmas were studied to corroborate this data. Multivariate analysis and ToF-SIMS are useful tools for quantitative compositional analysis of multicomponent adsorbed protein films. @FootnoteText@ @footnote 1@ M. S. Wagner and D. G. Castner, Langmuir in press (2001).