AVS 47th International Symposium
    MEMS Thursday Sessions
       Session MM+BI-ThM

Paper MM+BI-ThM3
Suppression of Blood Serum Adhesion on Quartz Inner Wall of Microcapillary Coated by Bio-compatible MPC Polymer

Thursday, October 5, 2000, 9:00 am, Room 309

Session: Bio-MEMS and Microfluidics
Presenter: Y. Takamura, The University of Tokyo, Japan
Authors: H. Ogawa, The University of Tokyo, Japan
A. Oki, The University of Tokyo, Japan
Y. Takamura, The University of Tokyo, Japan
S. Adachi, The University of Tokyo, Japan
T. Ichiki, Toyo University, Japan
K. Ishihara, The University of Tokyo, Japan
Y. Horiike, The University of Tokyo, Japan
Correspondent: Click to Email
We are developing the healthcare device which enables us to detect various health markers from trace amount of the blood. To suppress adhesion of proteins in a blood injected into a quartz made microcapillary, the 2-methacryloyloxyethylphosphorylcholine (MPC) polymer which is now utilized to artificial blood tube, contact lens, in-vivo sensors, etc. has been coated on the inner wall, thus leading to bio-compatibility of the quartz surface. A microcapillary with a 30 x 30 µm cross-section and 10 mm in length was fabricated in a 2cm x 2cm quartz chip by dry-etching and subsequent press-bonding of a cover quartz plate in 1% HF solution. A pH=7.4 phosphate buffered solution (PBS) was filled in the capillary and the serum was injected from one end by electroosmosis pumping. The concentrations of proteins, which were monitored by 220nm UV absorption at the point of 4mm apart from the injection, rose up rapidly when the serum arrived at this point and then were kept at a constant value in the 3 wt% MPC coated capillary, while those in uncoated capillary were decreased gradually by adhesion of proteins on the inner wall after showed maximum. To investigate the interaction of proteins with the MPC polymer coated surface, FTIR-ATR spectra for MPC coated and uncoated wall surfaces exposed by a blood serum were measured. The adsorption peaks by NH@sub x@ and C=O of proteins in the uncoated surface increased with exposure time, while the proteins did not absorb on the coated surface. These results clearly demonstrate the excellent bio-compatiblity of the MPC polymer for a blood handling capillary chip.