AVS 47th International Symposium
    Biomaterial Interfaces Tuesday Sessions
       Session BI-TuM

Paper BI-TuM11
The Molecular Orientation Distribution of an Electrochemically Active Protein Monolayer Adsorbed to Indium-Tin Oxide

Tuesday, October 3, 2000, 11:40 am, Room 202

Session: Protein-Surface Interactions
Presenter: R.T. Robertson, University of Arizona
Authors: S. Saavedra, University of Arizona
R.T. Robertson, University of Arizona
S.B. Mendes, University of Arizona
N.R. Armstrong, University of Arizona
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The relationship between molecular orientation and heterogeneous electron transfer behavior in immobilized films of redox-active proteins is being investigated using absorbance and fluorescence techniques that combine the information content of spectroele ctrochemistry with the sensitivity of the single-mode, planar waveguide geometry. Spectroelectrochemistry of surface confined, redox-active films can be performed with a pathlength enhancement of approximately 4,000 relative to a transmission geometry. T he use of this approach to determine the tilt angle distribution of the porphyrin molecular planes in a submonolayer of electrochemically active cytochrome c adsorbed to an indium-tin oxide electrode will be described. Developing a better understanding of the relationship between protein film structure and redox activity may aid efforts to rationally design protein-based molecular devices in which control of vectorial electron transfer is a prerequisite for efficient operation.