AVS 47th International Symposium
    Biomaterial Interfaces Friday Sessions
       Session BI-FrM

Paper BI-FrM7
Immobilized Antibodies on Functionalized Self-assembled Monolayers: Reactivity, Surface Homogeneity and Microarraying

Friday, October 6, 2000, 10:20 am, Room 202

Session: Biomolecular Recognition at Surfaces
Presenter: P. Kernen, Zyomyx Inc.
Authors: P. Kernen, Zyomyx Inc.
F. Zaugg, Zyomyx Inc.
K. Witte, Zyomyx Inc.
D. Quincy, Zyomyx Inc.
P. Indermuehle, Zyomyx Inc.
S. Nock, Zyomyx Inc.
P. Wagner, Zyomyx Inc.
Correspondent: Click to Email
@omega@-Substituted alkanethiols self-assemble in ordered monolayers on Au(111) surfaces and form highly reproducible reactive interfaces for biomolecule immobilization. Reaction conditions for covalent coupling of antibodies to monolayers exposing N-hydroxysuccinimide and other functionalities have been tested using radiometry and fluorescence microscopy. Scanning probe microscopy, X-ray photoelectron spectroscopy and other surface analytical techniques have been applied to characterize homogeneity and surface coverage of covalently bound biomolecules. By incorporating these bioreactive interfaces into microfabricated three-dimensional structures in silicon, functional microarrays of antibodies could be produced with variable feature size.