AVS 47th International Symposium
    Biomaterial Interfaces Tuesday Sessions
       Session BI+EL-TuA

Paper BI+EL-TuA3
Cell Respone to Chemically and Topographically Modified Surfaces

Tuesday, October 3, 2000, 2:40 pm, Room 202

Session: Cell-Surface Interactions
Presenter: D.S. Sutherland, Chalmers University of Technology, Sweden
Authors: D.S. Sutherland, Chalmers University of Technology, Sweden
A.S. Andersson, Chalmers University of Technology, Sweden
K. Glasmastar, Chalmers University of Technology, Sweden
S. Petronis, Chalmers University of Technology, Sweden
F. Backhed, Karolinska Institute, Sweden
A. Richter-Dahlfors, Karolinska Institute, Sweden
U. Lidberg, University of Gothenburg, Sweden
B. Kasemo, Chalmers University of Technology, Sweden
Correspondent: Click to Email
The properties of surfaces have long been known to influence cellular behaviour. Both the chemistry and topography of surfaces have been shown to effect different aspects of cellular response. With the advent of micro and nanofabrication it is now possibl e to study these interaction in a more detailed fashion, isolating specific surface structures and systematically varying their size and shape. In a parallel multicentre project a range of micro and nanofabricated surfaces are used in cell culture experi ments with a range of cell types. The specific surface designs were selected to give chemical and topographic cues on a range of length scales from the micron and submicron to the nanometre and were used as a set, to screen for the influence of surface st ructure on cellular behaviour. Similar sets of well-characterised surfaces were used in a number of different cell culture systems, including epithelial, endothelial, mammary gland and pancreatic cells, to look for both cell-specific interactions and g ene ric correlations. The studies have taken advantage of recent advances in microbiological techniques, focussing on different aspects of gene expression, cell differentiation and cell-cell signalling as well as more traditional adhesion, proliferation and m orphologic analysis. Examples of preliminary results obtained so far include: 1. Non-adherence/proliferation of three cell types to lipid bilayers (so called supported membranes) 2. Expression of a specific cytokine by epithelial cells is influenced by the microtopography of the surface. Additional results from ongoing studies are expected within a few months.