AVS 46th International Symposium
    Biomaterial Interfaces Group Wednesday Sessions
       Session BI-WeM

Paper BI-WeM5
Biocompatibility of Cardiac Cells on Silane-Modified Surfaces

Wednesday, October 27, 1999, 9:40 am, Room 613/614

Session: Cell Solid-Surface Interactions
Presenter: J.J. Hickman, The George Washington University
Authors: J.J. Hickman, The George Washington University
M.S. Ravenscroft, The George Washington University
H. Canavan, The George Washington University
V. Krauthamer, Food and Drug Administration
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We are investigating the interaction of cardiac cells with select silane-modified surfaces in a defined culture system. Our goal is to examine cell reaction in vitro to the types of modified surfaces that may be useful as implant coatings with an assay that we could relate to organ function. The SAM-modified glass coverslips were analyzed using X-ray Photoelectron Spectroscopy (XPS). The morphology was determined by image analysis and the excitability of the cultured cardiac cells was examined electrophysiologically by Calcium imaging both at rest and while being stimulated. Stimulation experiments electrically pace the cells at select time intervals and monitor the resulting changes in intracellular [Ca]. We observed a significant difference in excitation-induced Ca changes on the different silanated surfaces without corresponding differences in cell morphology. This result implies a change in cardiac Ca channel function on SAMs with different functional groups that would not be detected by morphological analysis alone.