AVS 46th International Symposium
    Biomaterial Interfaces Group Tuesday Sessions
       Session BI-TuP

Paper BI-TuP20
Solid Supported Lipid Bilayers by Fusion of Mixed Thiolipid/Lipid Vesicles onto Gold

Tuesday, October 26, 1999, 5:30 pm, Room 4C

Session: Poster Session
Presenter: H. Wieder, Max-Planck-Institute for Polymer Research, Germany
Authors: H. Wieder, Max-Planck-Institute for Polymer Research, Germany
S. Lingler, Max-Planck-Institute for Polymer Research, Germany
J. Mack, Institute for Organic Chemistry, Germany
A. Offenhäusser, Max-Planck-Institute for Polymer Reseach, Germany
Correspondent: Click to Email
Recently, planar solid supported lipid membranes have become a frequently used model system for biological membranes. Their big advantage to other model systems is the relatively high stability to mechanical and electrochemical stress in addition to the accessibility by a variety of surface sensitive techniques. These features make them an interesting system for biosensing applications. In this work supported lipid mono- and bilayers tethered to Au-electrodes by thiolipids are being investigated using surface plasmon resonance spectroscopy (SPR), impedance spectroscopy (IS), cyclic voltammetry (CV), reductive desorption and contact angle measurements. Following work on hybrid bilayer membranes,@footnote 1@ we are now looking at systems with and without laterally diluted hydrophilic spacers of different length to provide a hydrophilic reservoir between membrane and support. The dependence of the membrane properties on different parameters in the preparation by self-assembly, LB-techniques and vesicle fusion are being investigated. Into the membranes noted above transmembrane proteins are reconstituted and checked for their functional activity. @FootnoteText@ @footnote 1@Lingler S., Rubinstein I., Knoll W., Offenhäusser A. (1997) Fusion of Small Unilamellar Lipid Vesicles to Alkanethiol and Thiolipid Self-Assembled Monolayers on Gold, Langmuir 13, 7085-7091.