AVS 46th International Symposium
    Biomaterial Interfaces Group Tuesday Sessions
       Session BI-TuP

Paper BI-TuP2
Immobilization of Proteins on Self-Assembled Monolayers by Electrostatic Interactions

Tuesday, October 26, 1999, 5:30 pm, Room 4C

Session: Poster Session
Presenter: Y. Du, University of Arizona
Authors: Y. Du, University of Arizona
S.S. Saavedra, University of Arizona
Correspondent: Click to Email
Immobilization of proteins onto solid substrates has been studied widely because of the importance of biomolecular films molecular devices. Adsorption of protein molecules onto solid surfaces by physical interactions is technically simple immobilization methods. However, in order to form highly ordered protein films, a single type of site-directed binding must be employed. In this study, sulfonated silane films were prepared on Si substrates. The resulting anionic surface was used to electrostatically adsorb positively charged proteins, such as horse heart cytchrome c. The formation of the sulfonated silane films was supported by XPS measurements, contact angle measurements, and ellipsometry measurements. The nature of the electrostatic adsorption of protein molecules and the protein film structure were investigated using a variety of surface spectroscopic techniques.