AVS 46th International Symposium
    Biomaterial Interfaces Group Tuesday Sessions
       Session BI-TuP

Paper BI-TuP19
Force Differentiation Assay: A New Approach Sensing

Tuesday, October 26, 1999, 5:30 pm, Room 4C

Session: Poster Session
Presenter: M. Natesan, GeoCenters
Authors: M. Natesan, GeoCenters
C. Yanavich, Nova Research
S. Metzger, Geocenters
G.U. Lee, Naval Research Laboratory
Correspondent: Click to Email
Biomolecular interactions have recently been measured at the single molecule scale using microscopic techniques such as atomic force microscopy, optical tweezers and micropipettes. The force required to rupture a specific interaction has been found to be a characteristic of the structure of the molecular interaction and the rate at which it is stressed. We have used these insights to develop a new approach to molecular detection based on sensing force. Force detection has two advantages, it has single molecule sensitivity and the magnitude of the force can be used to differentiate specific from nonspecific interactions. We will describe two force based diagnostic techniques that apply a magnetically derived force to an antibody-antigen sandwich using a micron size particle.