AVS 46th International Symposium
    Biomaterial Interfaces Group Tuesday Sessions
       Session BI-TuP

Paper BI-TuP12
Human Monocyte/Macrophage Adhesion to Biomaterials Surfaces

Tuesday, October 26, 1999, 5:30 pm, Room 4C

Session: Poster Session
Presenter: J. Shan, University of Washington
Authors: J. Shan, University of Washington
T.A. Horbett, University of Washington
C.M. Giachelli, University of Washington
Correspondent: Click to Email
The biocompatibility of synthetic implants is thought to be related to their attractiveness to monocyte/macrophages. The hydrophobicity and protein adsorption capabilities of an implant surface are believed to influence the adhesion of monocytes and macro phages. Therefore, a range of materials varying widely in hydrophobicity and protein adsorption characteristics were evaluated for monocyte/macrophage adhesion in vitro. Freshly isolated human peripheral blood monocytes were cultured on surfaces in the p r esence of autologous serum from 2 hour up to 8 days. The surfaces includes polystyrene (PS), tissue culture treated polystyrene(TCPS), human fibrinogen (Fbg) preadsorbed to PS (Fbg-PS), BSA preadsorbed PS (BSA-PS), plasma polymerized hexaflurocarbon (C 3F6) coated PS, plasma polymerized triglyme coated PS(triglyme), fluoro-ethylene-propylene (FEP) copolymer, and plasma polymerized tetraglyme coated FEP(PEO-FEP). Monocyte adhesion was determined by measuring the LDH activity of lysates. Of these surfaces, t he most hydrophobic is C3F6 coated PS, the most hydrophilic surface was PEO-FEP, which had less than 5ng/cm2 adsorption of Fbg from 1% human plasma. Adhesion to most PS based surfaces was similar for periods up to 24 hours. However, monocyte adhesion to T CPS was much lower by 24 hours. Monocyte adhesion to PEO-FEP at earlier hours was about 1/2 to 1/3 of the level achieved on PS and FEP, respectively. After the 8 day period, macrophage adhesion to the surfaces ranged from none on TCPS and Fbg-PS, low on B SA-PS, to significantly higher on PS, C3F6, triglyme and PEO-FEP. Adhesion to PEO-FEP after the 8-day period was similar to that on PS, C3F6, and triglyme, however macrophage morphologies differed among surfaces. Possible explanations (including non-protein mediated mechanisms) for monocyte/macrophage adhesion to PEO-film will be included in the poster presentation.