AVS 46th International Symposium
    Biomaterial Interfaces Group Tuesday Sessions
       Session BI-TuM

Paper BI-TuM5
Protein Adsorption Kinetics: Particle Model and Optical Experiment

Tuesday, October 26, 1999, 9:40 am, Room 613/614

Session: Protein Solid-Surface Interactions II
Presenter: P.R. Van Tassel, Wayne State University
Authors: M.A. Brusatori, Wayne State University
C. Calonder, Wayne State University
P.R. Van Tassel, Wayne State University
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The adsorption of proteins at the liquid-solid interface is a phenomenon of great importance in bioseparations, biocatalysis, and materials biocompatibility. Adsorption is often accompanied by a surface-induced transition in either internal conformation or molecular orientation. Recently, Van Tassel, et al, presented a model in which the adsorption/ transition process is modeled as the sequential surface placement of spreading disks. In this talk, we present a derivation of analytical expressions for the adsorption and spreading probabilities, whose use greatly simplyfies the form of the kinetic equations for this model, using the scaled particle theory (SPT). We also present new experimental data of fibrinogen adsorption onto SiTiO2 and dextran-coated SiTiO2 using optical waveguide lightmode spectroscopy (OWLS). We show that the SPT spreading disk model can accurately predict these and other experimental data as well as those from computer simulation.